{"id":13476,"date":"2008-10-06T12:59:43","date_gmt":"2008-10-06T12:59:43","guid":{"rendered":"http:\/\/www.cienciasdasaude.org\/portal\/?p=13476"},"modified":"2025-02-10T02:36:21","modified_gmt":"2025-02-10T02:36:21","slug":"tecnica-de-coloracao-de-papanicolau","status":"publish","type":"post","link":"https:\/\/antonini.psc.br\/?p=13476","title":{"rendered":"T\u00e9cnica de colora\u00e7\u00e3o de papanicolau"},"content":{"rendered":"<ul>\n<li>Fixar os \tesfrega\u00e7os em \u00e1lcool absoluto.<\/li>\n<\/ul>\n<ol>\n<table border=\"1\" cellspacing=\"0\" cellpadding=\"4\" width=\"100%\">\n<col width=\"128*\"\/>\n<col width=\"128*\"\/>\n<tbody>\n<tr valign=\"TOP\">\n<td style=\"text-align: center;\" width=\"50%\"><strong><em>Corantes \/ reagentes<\/em><\/strong><\/td>\n<td style=\"text-align: center;\" width=\"50%\"><strong><em>Tempo \/ mergulhos<\/em><\/strong><\/td>\n<\/tr>\n<tr valign=\"TOP\">\n<td width=\"50%\">1. \u00c1lcool 80% (+ ou -)<\/td>\n<td width=\"50%\">15 mergulhos<\/td>\n<\/tr>\n<tr valign=\"TOP\">\n<td width=\"50%\">2. \u00c1lcool 70%<\/td>\n<td width=\"50%\">15 mergulhos<\/td>\n<\/tr>\n<tr valign=\"TOP\">\n<td width=\"50%\">3. \u00c1lcool 50%<\/td>\n<td width=\"50%\">15 mergulhos<\/td>\n<\/tr>\n<tr valign=\"TOP\">\n<td width=\"50%\">4. \u00c1gua destilada<\/td>\n<td width=\"50%\">05 mergulhos<\/td>\n<\/tr>\n<tr valign=\"TOP\">\n<td width=\"50%\">5. Hematoxilina de Harris<\/td>\n<td width=\"50%\">02 <strong>minutos<\/strong><\/td>\n<\/tr>\n<tr valign=\"TOP\">\n<td width=\"50%\">6. \u00c1gua corrente<\/td>\n<td width=\"50%\">para tirar o excesso de corante<\/td>\n<\/tr>\n<tr valign=\"TOP\">\n<td width=\"50%\">7. Solu\u00e7\u00e3o aquosa de Hcl a 0,25%<\/td>\n<td width=\"50%\">02 mergulhos <strong>r\u00e1pidos<\/strong><\/td>\n<\/tr>\n<tr valign=\"TOP\">\n<td width=\"50%\">8. \u00c1gua corrente<\/td>\n<td width=\"50%\">10 minutos aproximadamente<\/td>\n<\/tr>\n<tr valign=\"TOP\">\n<td width=\"50%\">9. \u00c1gua destilada<\/td>\n<td width=\"50%\">10 <strong>minutos<\/strong><\/td>\n<\/tr>\n<tr valign=\"TOP\">\n<td width=\"50%\">10. \u00c1lcool 50%<\/td>\n<td width=\"50%\">15 mergulhos<\/td>\n<\/tr>\n<tr valign=\"TOP\">\n<td width=\"50%\">11. \u00c1lcool 70%<\/td>\n<td width=\"50%\">15 mergulhos<\/td>\n<\/tr>\n<tr valign=\"TOP\">\n<td width=\"50%\">12. \u00c1lcool 80%<\/td>\n<td width=\"50%\">15 mergulhos<\/td>\n<\/tr>\n<tr valign=\"TOP\">\n<td width=\"50%\">13. Orange C-6<\/td>\n<td width=\"50%\">1 <strong>minuto<\/strong><\/td>\n<\/tr>\n<tr valign=\"TOP\">\n<td width=\"50%\">14. \u00c1lcool absoluto<\/td>\n<td width=\"50%\">15 mergulhos<\/td>\n<\/tr>\n<tr valign=\"TOP\">\n<td width=\"50%\">15. \u00c1lcool absoluto<\/td>\n<td width=\"50%\">15 mergulhos<\/td>\n<\/tr>\n<tr valign=\"TOP\">\n<td width=\"50%\">16. \u00c1lcool absoluto<\/td>\n<td width=\"50%\">15 mergulhos<\/td>\n<\/tr>\n<tr valign=\"TOP\">\n<td width=\"50%\">17. EA 36<\/td>\n<td width=\"50%\">05 <strong>minutos<\/strong><\/td>\n<\/tr>\n<tr valign=\"TOP\">\n<td width=\"50%\">18. \u00c1lcool absoluto<\/td>\n<td width=\"50%\">15 mergulhos<\/td>\n<\/tr>\n<tr valign=\"TOP\">\n<td width=\"50%\">19. \u00c1lcool absoluto<\/td>\n<td width=\"50%\">15 mergulhos<\/td>\n<\/tr>\n<tr valign=\"TOP\">\n<td width=\"50%\">20. \u00c1lcool absoluto<\/td>\n<td width=\"50%\">15 mergulhos<\/td>\n<\/tr>\n<tr valign=\"TOP\">\n<td width=\"50%\">21. \u00c1lcool absoluto<\/td>\n<td width=\"50%\">15 mergulhos<\/td>\n<\/tr>\n<tr valign=\"TOP\">\n<td width=\"50%\">22. \u00c1lcool absoluto \/ xilol<\/td>\n<td width=\"50%\">10 mergulhos<\/td>\n<\/tr>\n<tr valign=\"TOP\">\n<td width=\"50%\">23. 1\u00ba Xilol<\/td>\n<td width=\"50%\">10 mergulhos<\/td>\n<\/tr>\n<tr valign=\"TOP\">\n<td width=\"50%\">24. 2\u00ba Xilol<\/td>\n<td width=\"50%\">10 mergulhos<\/td>\n<\/tr>\n<tr valign=\"TOP\">\n<td width=\"50%\">25. 3\u00ba Xilol<\/td>\n<td width=\"50%\">10 mergulhos<\/td>\n<\/tr>\n<tr valign=\"TOP\">\n<td width=\"50%\">26. 4\u00ba Xilol de montagem<\/td>\n<td width=\"50%\">10 <strong>minutos<\/strong><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/ol>\n<p><strong>=&gt; <\/strong>Montar a l\u00e2mina com a \tlam\u00ednula e B\u00e1lsamo do Canad\u00e1.<\/p>\n<p>Cuidados:<\/p>\n<ol>\n<li>Filtrar \tdiariamente <span style=\"text-decoration: underline;\"><strong>antes<\/strong><\/span> do uso: <strong>Hematoxilina<\/strong>.<\/li>\n<li>Filtar diariamente \t<span style=\"text-decoration: underline;\"><strong>depois<\/strong><\/span> do uso: <strong>Xilol \tde montagem<\/strong><\/li>\n<li>Filtar \tdiariamente<strong> <\/strong><span style=\"text-decoration: underline;\"><strong>depois<\/strong><\/span> do uso: <strong>\u00c1lcool \tabsoluto.<\/strong><\/li>\n<li>Filtrar semanalmente os demais \tcorantes.<\/li>\n<\/ol>\n<p style=\"text-align: center;\">[<a href=\"javascript:history.go(-1)\">Voltar<\/a>]<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Fixar os esfrega\u00e7os em \u00e1lcool absoluto. Corantes \/ reagentes Tempo \/ mergulhos 1. \u00c1lcool 80% (+ ou -) 15 mergulhos 2. \u00c1lcool 70% 15 mergulhos 3. \u00c1lcool 50% 15 mergulhos 4. \u00c1gua destilada 05 mergulhos 5. Hematoxilina de Harris 02 minutos 6. \u00c1gua corrente para tirar o excesso de corante 7. Solu\u00e7\u00e3o aquosa de Hcl [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[81],"tags":[],"class_list":["post-13476","post","type-post","status-publish","format-standard","hentry","category-citologia"],"_links":{"self":[{"href":"https:\/\/antonini.psc.br\/index.php?rest_route=\/wp\/v2\/posts\/13476","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/antonini.psc.br\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/antonini.psc.br\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/antonini.psc.br\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/antonini.psc.br\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=13476"}],"version-history":[{"count":1,"href":"https:\/\/antonini.psc.br\/index.php?rest_route=\/wp\/v2\/posts\/13476\/revisions"}],"predecessor-version":[{"id":30736,"href":"https:\/\/antonini.psc.br\/index.php?rest_route=\/wp\/v2\/posts\/13476\/revisions\/30736"}],"wp:attachment":[{"href":"https:\/\/antonini.psc.br\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=13476"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/antonini.psc.br\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=13476"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/antonini.psc.br\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=13476"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}